Student Reflection - Grayson Means

The role of B56a and B56d/g in convergent extension

The summer of 2014 was a productive several months for my research project. The first week was spent outlining what I would be doing for the following months. This step was important so that I was able to efficiently use those few summer weeks. Following that, I began practicing the techniques to be implemented in my experiment. Besides general lab skills involving mixing and preparing solutions, the two primary techniques I practiced during this time were microinjection and microdissection.

These techniques are very commonly used in the study of Xenopus embryonic development. Microinjection is a technique wherein small amounts of RNA, which are blueprints for proteins, are injected into a live Xenopus embryo. This technique allows us to observe how these proteins act within a developing organism. Microdissection is a technique that is most commonly used after microinjection has been performed. Microdissection is used to remove a specific region of tissue from a developing embryo. It is extremely useful for observing how a specific region of tissue develops, and how injecting or blocking RNA in the embryo affects that particular region.

The summer months allowed me to regularly practice these techniques, which is the absolute best way to learn them. Through routine practice, I was able to gain a firm grasp of both microinjection and microdissection. The opportunity granted to me by the FAST program was invaluable. It allowed me to fully focus on conducting research without the distractions of work or classes. This allowed me, for the first time in my life, to gain a view into what it is like to only work on my research. As an undergraduate, this is a rare opportunity. I am extremely grateful to all of those involved in the FAST program. My experiences this summer have helped me on my path as a future scientist and doctor.